immunohistochemistry

listen to the pronunciation of immunohistochemistry
Englisch - Englisch
The analytical process of finding proteins in cells of a tissue microtome section exploiting the principle of antibodies binding specifically to antigens in biological tissues
(Tıp, İlaç) An assay that shows specific antigens in tissues by the use of markers that are either fluorescent dyes or enzymes such as horseradish peroxidase
Chemical techniques used to indentify the deposit of particular chemicals or proteins in tissue Common used to locate prion protein in brain tissue from SE using an anti-PrP antibody
A method of detecting the presence of specific proteins in cells or tissues Fixed cells or tissue on a microscope slide, made permeable if necessary with a detergent, are reacted with a primary antibody to the specific protein to be assayed The preparation is then treated with a secondary antibody that has been coupled to an enzyme and which is directed against the primary antibody (e g , goat anti-rabbit antibody) The preparation is then treated with a chromogenic substrate Microscopic examination reveals the presence of staining, and hence of the specific protein to be detected See one of the Figures from GXD for an example
n Process that involves treating a part of a biopsy sample with special laboratory antibodies that recognize cell proteins typical of certain kinds of cancers The cells are treated with additional chemicals that cause the cells containing the specific proteins to change color, which can be detected under a microscope
Chemical techniques used to identify, under the microscope, particular chemicals or proteins in tissue Commonly used to locate prion protein in brain tissue from SE using and anti-PrP antibody
The testing of sections of tissue for specific proteins by attatching them with specific antibodies, and then looking for the antibodies through the enzymes to which they were connected
the application of antibody-antigen interactions to histochemical techniques, as in the use of immunofluorescence
an assay that shows specific antigens in tissues by the use of markers that are either fluorescent dyes or enzymes (such as horseradish peroxidase)
The testing of sections of tissue for specific proteins by attaching them with specific antibodies, and then looking for the antibodies through the enzymes to which they were connected
The use of antibodies or antisera as histological tools for identifying patterns of antigen distribution within a tissue or an organism An antibody (or mixture of antibodies) that binds to a specific protein or other antigen is tagged with a fluorescent chemical or an enzyme that can convert a substrate to a visible dye The tagged antibody is incubated with the tissue and after washing unbound antibody away the bound antibody distribution is revealed by fluorescence microscopy or incubation with a chromogenic substrate It is assumed that antibody distribution reflects antigen distribution